Comparative analysis of the effects of retinoic acid versus paclitaxel and everolimus on HL60 cells proliferation and viability

Δημοσίευση στο BioRxiv:

 

Comparative analysis of the effects of retinoic acid versus paclitaxel and everolimus on HL60 cells proliferation and viability. Athanasia Zampouka, Triantafyllia Papadimitropoulou, Maria Salagianni, Maria Vaiou, Amalia I Moula, Athanasios D Giannoukas, Anargyros N Moulas bioRxiv 2023.04.03.535342; doi:

https://doi.org/10.1101/2023.04.03.535342

https://www.biorxiv.org/content/10.1101/2023.04.03.535342v1,

19th International Conference on Bioinformatics and Bioengineering (BIBE) 2019

Δημοσίευση στα πρακτικά του διεθνούς συνεδρίου 19th International Conference on Bioinformatics and Bioengineering (BIBE) 2019.

 

BioCoStent: A Holistic Approach for Development of a Drug-Eluting Stent with Retinoic Acid

Georgia Karanasiou;Savvas Kyriakidis;Dimitris Pleouras;Antonis Sakellarios;Anargyros Moulas;Arsen Semertzioglou;Dimitrios Fotiadis

2019 IEEE 19th International Conference on Bioinformatics and Bioengineering (BIBE). DOI: 10.1109/BIBE.2019.00125

 

https://ieeexplore.ieee.org/author/37087135578

 

Abstract:

Coronary artery disease (CAD) is one of the leading causes of mortality worldwide. Drug-eluting stents (DES) are nowadays widely used so as to treat the occluded arteries, restore blood flow and through the diffusion of the drug achieve better clinical outcomes compared to Bare Metal Stents (BMSs), in terms of reduced numbers of cardiac death, myocardial infarction and vessel revascularization. BioCoStent targets the design and development of an innovative DES with retinoic acid. In this study the overall concept for realizing this new DES development, including the characterization of the biomaterials, the performance of in vivo and in vitro studies and the optimisation through in silico modelling, is presented.

Investigation of Drug Eluting Stents performance in human atherosclerotic artery through in silico modeling

Abstract:

Atherosclerosis is a chronic inflammatory disease associated with heart attack and stroke. It causes the growth of atherosclerotic plaques inside the arterial vessels, which in turn results to the reduction of the blood flow to the different organs. Drug-Eluting Stents (DES) are mesh-like wires, carrying pharmaceutical coating, designed to dilate and support the arterial vessel, restore blood flow and through the controlled local drug delivery inhibit neo-intimal thickening. In silico modeling is an efficient method of accurately predicting and assessing the performance of the stenting procedure. The present in silico study investigates the performance of two different stents (Bare Metal Stent, Drug-Eluting Stent) in a patient-specific coronary artery and assesses the effect of stent coating, considering that the same procedural approach is followed by the interventional cardiologist. The results demonstrate that even if small differences are obtained in the two models, the incorporation of the stent coatings (in DES) does not significantly affect the outcomes of the stent deployment, the stresses and strains in the scaffold and the arterial tissue. Nevertheless, it is suggested that regarding the DES expansion, higher pressure should be applied at the inner surface of the stent.
Date of Conference: 01-05 November 2021
Date Added to IEEE Xplore: 09 December 2021
ISBN Information:
ISSN Information:
PubMed ID: 34892355
INSPEC Accession Number: 21385672
Publisher: IEEE

Conference Location: Mexico

Loukas, V. S., Karanasiou, G. S., Pleouras, D., Kyriakidis, S., Sakellarios, A. I., Semertzioglou, A., Michalis, L. K., & Fotiadis, D. I. (2021). Investigation of Drug Eluting Stents performance in human atherosclerotic artery through in silico modeling. Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference, 2021, 5433–5436.

FEA of Drug-Eluting Stents and Sensitivity Analysis of a Continuum Damage Model for the Degradation of PLGA Coating

Abstract:

Drug-Eluting Stents (DES) are commonly used in coronary angioplasty operations as a solution against artery stenosis and restenosis. Computational Bioengineering allows for the in-silico analysis of their performance. The scope of this work is to develop a DES Digital Twin, focusing on the mechanical integrity of its biodegradable coating throughout the operational lifecycle. The implementation leverages the Finite Element Method (FEM) to compute the developed mechanical stress field on the DES during the inflation/deflation stage, followed by the degradation of the polymer-based coating. The simulation of the degradation process is based on a Continuum Damage Mechanics (CDM) model that considers bulk degradation. The CDM algorithm is implemented on the NX Nastran solver through a user-defined material (UMAT) subroutine. For benchmarking purposes and to compare with the baseline design of the BioCoStent project, this conceptual study implements an alternative stent design, to study the effect of the geometry on the developed stresses. Additionally, the effect of the degradation rate on the polymer-based coating’s lifecycle is studied via sensitivity analysis.
Date of Conference: 01-05 November 2021
Date Added to IEEE Xplore: 09 December 2021
ISBN Information:
ISSN Information:

PubMed ID: 34892178

Kokkinos, C., Drakoulas, G., Fotiadis, D., Kokkinos, S., Loukas, K., Moulas, A. N., & Semertzioglou, A. (2021). FEA of Drug-Eluting Stents and Sensitivity Analysis of a Continuum Damage Model for the Degradation of PLGA Coating. Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference, 2021, 4324–4328.

Coupled FEA Model with Continuum Damage Mechanics for the Degradation of Polymer-based coatings on Drug-Eluting Stents

Abstract:

Drug-Eluting Stents (DES) are commonly used in Coronary angioplasty procedures to reduce the phenomenon of restenosis. Numerical simulations are proven to be a useful tool to the Bioengineering community in computing the mechanical performance of stents. BioCoStent is a research project aiming to develop a DES with retinoic acid (RA) coating, in the frame of which FEAC is responsible for the in silico numerical simulation of the coating’s degradation in terms of Finite Element Analysis (FEA). The coatings under study are poly(lactic-co-glycolic acid) (PLGA) and polylactide (PLA). The FEA is based on the Continuum Damage Mechanics (CDM) theory and considers a mechanistic model for polymer bulk degradation of the coatings. The degradation algorithm is implemented on the NX Nastran solver through a user-defined material UMAT subroutine. This paper describes the developed numerical model to compute the degradation of biodegradable coatings on DES. The transient numerical model provides useful insight into the critical areas with regards to the scalar damage of the coatings. The FEA results present a complete degradation of polymers after several weeks.
Date of Conference: 01-05 November 2021
Date Added to IEEE Xplore: 09 December 2021
ISBN Information:
ISSN Information:

PubMed ID: 34892177

Drakoulas, G., Kokkinos, C., Fotiadis, D., Kokkinos, S., Loukas, K., Moulas, A. N., & Semertzioglou, A. (2021). Coupled FEA Model with Continuum Damage Mechanics for the Degradation of Polymer-based coatings on Drug-Eluting Stents. Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference, 2021, 4319–4323.

Investigation of the drug release time from the biodegrading coating of an everolimus eluting stent

Abstract:

This case-study examines the release time of the everolimus drug from an experimental biodegrading coating of a Rontis corp. drug eluting stent (DES). The controlled drug release is achieved by the degradation of the coating, which consists of a mixture of polylactic co-glycolic acid (PLGA) and everolimus (55:45). In our analysis, we used the outcome of another study, which contains the geometry of an in-silico deployed Rontis corp. stent in a 3D reconstructed coney arterial segment. Using this geometry as input, the everolimus release was simulated using a computational model that includes: i) modeling of the blood flow dynamics, ii) modeling of PLGA degradation, and iii) modeling of the everolimus advection and diffusion towards both the lumen and the arterial wall. The results show the rapid release of everolimus. This is justified due to the high porosity of the coating, which is caused by the initial high concentration of everolimus in the coating.Clinical Relevance — The methodology presented in this work is an additional step towards predicting accurately drug release from DES. Also, the results of our work prove that high drug concentration in the coating causes its rapid release, which could be used as input in the design of new DES.
Date of Conference: 01-05 November 2021
Date Added to IEEE Xplore: 09 December 2021
ISBN Information:
ISSN Information:
PubMed ID: 34891613
INSPEC Accession Number: 21386111
Publisher: IEEE

Conference Location: Mexico

Pleouras, D. S., Karanasiou, G. S., Loukas, V. S., Semertzioglou, A., Moulas, A. N., & Fotiadis, D. I. (2021). Investigation of the drug release time from the biodegrading coating of an everolimus eluting stent. Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference, 2021, 1698–1701.

BioCoStent leaflet

Δημοσιεύσεις BioCoStent Publications

 

  1. Kokkinos, C., Drakoulas, G., Fotiadis, D., Kokkinos, S., Loukas, K., Moulas, A. N., & Semertzioglou, A. (2021). FEA of Drug-Eluting Stents and Sensitivity Analysis of a Continuum Damage Model for the Degradation of PLGA Coating. Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference, 2021, 4324–4328. https://doi.org/10.1109/EMBC46164.2021.9630612 https://ieeexplore.ieee.org/document/9630612

 

 

  1. Drakoulas, G., Kokkinos, C., Fotiadis, D., Kokkinos, S., Loukas, K., Moulas, A. N., & Semertzioglou, A. (2021). Coupled FEA Model with Continuum Damage Mechanics for the Degradation of Polymer-based coatings on Drug-Eluting Stents. Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference, 2021, 4319–4323. https://doi.org/10.1109/EMBC46164.2021.9631074 https://ieeexplore.ieee.org/document/9631074

 

  1. Pleouras, D. S., Karanasiou, G. S., Loukas, V. S., Semertzioglou, A., Moulas, A. N., & Fotiadis, D. I. (2021). Investigation of the drug release time from the biodegrading coating of an everolimus eluting stent. Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference, 2021, 1698–1701. https://doi.org/10.1109/EMBC46164.2021.9629813 https://ieeexplore.ieee.org/document/9629813

 

  1. Loukas, V. S., Karanasiou, G. S., Pleouras, D., Katsouras, C. C., Tachos, N. S., Sakellarios, A. I., Semertzioglou, A., Michalis, L. K., & Fotiadis, D. I. (2022). Investigation of crimping effects on the stent deployment through in silico modeling. Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference, 2022, 621–624. https://doi.org/10.1109/EMBC48229.2022.9871622 https://ieeexplore.ieee.org/document/9871622

 

  1. Loukas, V. S., Karanasiou, G. S., Pleouras, D., Kyriakidis, S., Sakellarios, A. I., Semertzioglou, A., Michalis, L. K., & Fotiadis, D. I. (2021). Investigation of Drug Eluting Stents performance in human atherosclerotic artery through in silico modeling. Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference, 2021, 5433–5436. https://doi.org/10.1109/EMBC46164.2021.9629895 https://ieeexplore.ieee.org/document/9629895

 

  1. Samara, I., Katsouras, C. S., Semertzioglou, A., Vratimos, A., Moula, A. I., Dimitriou, C. A., Theofanis, M., Papadimitropoulou, T., Bouratzis, V., Karanasiou, G., Fotiadis, D., Michalis, L. K., & Moulas, A. N. (2022). Histopathological evaluation of a retinoic acid eluting stent in a rabbit iliac artery model. Scientific reports, 12(1), 13305. https://doi.org/10.1038/s41598-022-16025-5 https://www.nature.com/articles/s41598-022-16025-5

 

  1. Anargyros N Moulas, Christos S Katsouras, Ioanna Samara, Amalia I Moula, Triantafyllia Papadimitropoulou, Athanassios Vratimos, Arsen Semertzioglou, Maria Vaiou, Athanasia Zampouka, Maria Salagianni, Evangelos Andreakos, Lampros K Michalis. Retinoic acid eluting stents for the treatment of vascular stenosis. A novel use for an old drug. Eurobiotech Congress. Prague, Czechia, October 5-7, 2022. Invited Presentation. http://eurobiotech2022.eu/

 

  1. Anargyros Moulas, Christos S Katsouras, Ioanna Samara, Amalia I Moula, Triantafyllia Papadimitropoulou, Athanassios Vratimos, Arsen Semertzioglou, Maria Vaiou, Athanasia Zampouka, Savvas Papadopoulos, Lampros K Michalis. Comparative in vivo study of a retinoic acid stent with absorbable polymer. Eurobiotech Congress. Prague, Czechia, October 5-7, 2022. Invited Presentation. http://eurobiotech2022.eu/

 

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